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Expression,purification and characterization of recombinant canine FGF21 in Escherichia coli
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نویسنده
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zheng z. ,yang c. ,yin r. ,jiang j. ,he h. ,wang x. ,kan m. ,xiao y.
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منبع
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protein and peptide letters - 2016 - دوره : 23 - شماره : 9 - صفحه:785 -789
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چکیده
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The canine metabolic diseases,such as obesity and diabetes,have become a worldwide problem. fibroblast growth factor 21 (fgf21) is a potent regulator which has many biological functions relative to metabolism regulation. it suggests that fgf21 plays important roles in regulating canine metabolic diseases. to acquire the recombinant canine fgf21 (rcfgf21) in escherichia coli,the recombinant bacteria were induced by 0.5 mm iptg for 16 hours at 16 °c,and the rcfgf21 protein was purified by ni-nta. 8 mg rcfgf21 was acquired from one liter bacteria. the rcfgf21 protein has specific immunoblot reactivity against anti-fgf21 and anti-his antibody. the in vivo experimental result showed that rcfgf21 can significantly reduce plasma glucose of stz-induced diabetic mice. © 2016 bentham science publishers.
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کلیدواژه
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Escherichia coli; Plasma glucose; Purification; Recombinant canine FGF21
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آدرس
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department of biochemistry and molecular biology,college of basic medical science,jilin university,changchun, China, department of biochemistry and molecular biology,college of basic medical science,jilin university,changchun, China, department of orthopaedic surgery,china-japan union hospital of jilin university,changchun, China, school of clinical medicine,jilin university,changchun, China, department of biochemistry and molecular biology,college of basic medical science,jilin university,changchun, China, department of biochemistry and molecular biology,college of basic medical science,jilin university,changchun, China, department of biochemistry and molecular biology,college of basic medical science,jilin university,changchun, China, department of biochemistry and molecular biology,college of basic medical science,jilin university,changchun, China
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Authors
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