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Cell-free technologies for proteomics and protein engineering
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نویسنده
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ohashi h. ,miyamoto-sato e.
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منبع
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protein and peptide letters - 2016 - دوره : 23 - شماره : 9 - صفحه:819 -827
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چکیده
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Cell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. moreover,these systems do not rely on living cells,thereby allowing the synthesis of unstable or cytotoxic proteins in vitro. in this review,we describe the principles and potential applications of cell-free protein translation systems and the future prospects of proteomics approaches using next-generation sequencing and cell-free expression technologies. © 2016 bentham science publishers.
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کلیدواژه
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Cell-free; IVV (in vitro virus); mRNA display; PPI (protein-protein interaction); Proteomics; PURE system
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آدرس
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division of interactome medical sciences,institute of medical science,university of tokyo,4-6-1 shirokanedai,minato-ku,tokyo, Japan, division of interactome medical sciences,institute of medical science,university of tokyo,4-6-1 shirokanedai,minato-ku,tokyo,japan,tokyo university of science research institute for biomedical science,division of molecular biology,2669 yamazaki,noda,chiba, Japan
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Authors
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