|
|
|
|
Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates
|
|
|
|
|
|
|
|
نویسنده
|
bar-yaacov d. ,frumkin i. ,yashiro y. ,chujo t. ,ishigami y. ,chemla y. ,blumberg a. ,schlesinger o. ,bieri p. ,greber b. ,ban n. ,zarivach r. ,alfonta l. ,pilpel y. ,suzuki t. ,mishmar d.
|
|
منبع
|
plos biology - 2016 - دوره : 14 - شماره : 9
|
|
چکیده
|
The mitochondrial ribosome,which translates all mitochondrial dna (mtdna)-encoded proteins,should be tightly regulated pre- and post-transcriptionally. recently,we found rna-dna differences (rdds) at human mitochondrial 16s (large) rrna position 947 that were indicative of post-transcriptional modification. here,we show that these 16s rrna rdds result from a 1-methyladenosine (m1a) modification introduced by trmt61b,thus being the first vertebrate methyltransferase that modifies both trna and rrnas. m1a947 is conserved in humans and all vertebrates having adenine at the corresponding mtdna position (90% of vertebrates). however,this mtdna base is a thymine in 10% of the vertebrates and a guanine in the 23s rrna of 95% of bacteria,suggesting alternative evolutionary solutions. m1a,uridine,or guanine may stabilize the local structure of mitochondrial and bacterial ribosomes. experimental assessment of genome-edited escherichia coli showed that unmodified adenine caused impaired protein synthesis and growth. our findings revealed a conserved mechanism of rrna modification that has been selected instead of dna mutations to enable proper mitochondrial ribosome function. © 2016 bar-yaacov et al.
|
|
|
|
|
آدرس
|
department of life sciences,ben-gurion university of the negev,beer sheva, Israel, department of molecular genetics,the weizmann institute of science,rehovot, Israel, department of chemistry and biotechnology,university of tokyo,tokyo, Japan, department of chemistry and biotechnology,university of tokyo,tokyo, Japan, department of chemistry and biotechnology,university of tokyo,tokyo, Japan, department of life sciences,ben-gurion university of the negev,beer sheva,israel,the ilse katz institute for nanoscale science and technology,beer sheva, Israel, department of life sciences,ben-gurion university of the negev,beer sheva, Israel, department of life sciences,ben-gurion university of the negev,beer sheva,israel,the ilse katz institute for nanoscale science and technology,beer sheva, Israel, department of biology,institute of molecular biology and biophysics,zurich, Switzerland, department of biology,institute of molecular biology and biophysics,zurich, Switzerland, department of biology,institute of molecular biology and biophysics,zurich, Switzerland, department of life sciences,ben-gurion university of the negev,beer sheva, Israel, department of life sciences,ben-gurion university of the negev,beer sheva,israel,the ilse katz institute for nanoscale science and technology,beer sheva, Israel, department of molecular genetics,the weizmann institute of science,rehovot, Israel, department of chemistry and biotechnology,university of tokyo,tokyo, Japan, department of life sciences,ben-gurion university of the negev,beer sheva, Israel
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|