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SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis
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نویسنده
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kim b.r. ,van de laar e. ,cabanero m. ,tarumi s. ,hasenoeder s. ,wang d. ,virtanen c. ,suzuki t. ,bandarchi b. ,sakashita s. ,pham n.a. ,lee s. ,keshavjee s. ,waddell t.k. ,tsao m.-s. ,moghal n.
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منبع
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plos biology - 2016 - دوره : 14 - شماره : 11
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چکیده
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Although cancers are considered stem cell diseases,mechanisms involving stem cell alterations are poorly understood. squamous cell carcinoma (sqcc) is the second most common lung cancer,and its pathogenesis appears to hinge on changes in the stem cell behavior of basal cells in the bronchial airways. basal cells are normally quiescent and differentiate into mucociliary epithelia. smoking triggers a hyperproliferative response resulting in progressive premalignant epithelial changes ranging from squamous metaplasia to dysplasia. these changes can regress naturally,even with chronic smoking. however,for unknown reasons,dysplasias have higher progression rates than earlier stages. we used primary human tracheobronchial basal cells to investigate how copy number gains in sox2 and pik3ca at 3q26-28,which co-occur in dysplasia and are observed in 94% of sqccs,may promote progression. we find that sox2 cooperates with pi3k signaling,which is activated by smoking,to initiate the squamous injury response in basal cells. this response involves sox9 repression,and,accordingly,sox2 and pi3k signaling levels are high during dysplasia,while sox9 is not expressed. by contrast,during regeneration of mucociliary epithelia,pi3k signaling is low and basal cells transiently enter a sox2losox9histate,with sox9 promoting proliferation and preventing squamous differentiation. transient reduction in sox2 is necessary for ciliogenesis,although sox2 expression later rises and drives mucinous differentiation,as sox9 levels decline. frequent coamplification of sox2 and pik3ca in dysplasia may,thus,promote progression by locking basal cells in a sox2hisox9lostate with active pi3k signaling,which sustains the squamous injury response while precluding normal mucociliary differentiation. surprisingly,we find that,although later in invasive carcinoma sox9 is generally expressed at low levels,its expression is higher in a subset of sqccs with less squamous identity and worse clinical outcome. we propose that early pathogenesis of most sqccs involves stabilization of the squamous injury state in stem cells through copy number gains at 3q,with the pro-proliferative activity of sox9 possibly being exploited in a subset of sqccs in later stages. © 2016 kim et al.
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آدرس
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princess margaret cancer centre,university health network,toronto,on,canada,department of medical biophysics,university of toronto,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on, Canada, division of thoracic surgery,university health network,university of toronto,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on,canada,helmholtz zentrum münchen,institute of stem cell research,neuherberg, Germany, princess margaret cancer centre,university health network,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on, Canada, division of thoracic surgery,university health network,university of toronto,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on,canada,department of applied mathematics,university of waterloo,waterloo,on, Canada, division of thoracic surgery,university health network,university of toronto,toronto,on, Canada, division of thoracic surgery,university health network,university of toronto,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on,canada,department of medical biophysics,university of toronto,toronto,on, Canada, princess margaret cancer centre,university health network,toronto,on,canada,department of medical biophysics,university of toronto,toronto,on, Canada
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Authors
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