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Cell Fate Regulation Governed by a Repurposed Bacterial Histidine Kinase
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نویسنده
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childers w.s. ,xu q. ,mann t.h. ,mathews i.i. ,blair j.a. ,deacon a.m. ,shapiro l.
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منبع
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plos biology - 2014 - دوره : 12 - شماره : 10
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چکیده
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One of the simplest organisms to divide asymmetrically is the bacterium caulobacter crescentus. the divl pseudo-histidine kinase,positioned at one cell pole,regulates cell-fate by controlling the activation of the global transcription factor ctra via an interaction with the response regulator (rr) divk. divl uniquely contains a tyrosine at the histidine phosphorylation site,and can achieve these regulatory functions in vivo without kinase activity. determination of the divl crystal structure and biochemical analysis of wild-type and site-specific divl mutants revealed that the divl pas domains regulate binding specificity for divk∼p over divk,which is modulated by an allosteric intramolecular interaction between adjacent domains. we discovered that divl's catalytic domains have been repurposed as a phosphospecific rr input sensor,thereby reversing the flow of information observed in conventional histidine kinase (hk)-rr systems and coupling a complex network of signaling proteins for cell-fate regulation. © 2014 childers et al.
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آدرس
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department of developmental biology,stanford university school of medicine,stanford,ca, United States, stanford synchrotron radiation lightsource,slac national accelerator laboratory,menlo park,ca,united states,joint center for structural genomics, United States, department of developmental biology,stanford university school of medicine,stanford,ca, United States, stanford synchrotron radiation lightsource,slac national accelerator laboratory,menlo park,ca, United States, department of developmental biology,stanford university school of medicine,stanford,ca,united states,department of chemistry,williams college,williamstown,ma, United States, stanford synchrotron radiation lightsource,slac national accelerator laboratory,menlo park,ca,united states,joint center for structural genomics, United States, department of developmental biology,stanford university school of medicine,stanford,ca, United States
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Authors
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