A single-strand annealing protein clamps DNA to detect and secure homology

Repair of dna breaks by single-strand annealing (ssa) is a major mechanism for the maintenance of genomic integrity. ssa is promoted by proteins (single-strand-annealing proteins [ssaps]),such as eukaryotic rad52 and λ phage redβ. these proteins use a short single-stranded region to find sequence identity and initiate homologous recombination. however,it is unclear how ssaps detect homology and catalyze annealing. using singlemolecule experiments,we provide evidence that homology is recognized by redβ monomers that weakly hold single dna strands together. once annealing begins,dimerization of redβ clamps the double-stranded region and nucleates nucleoprotein filament growth. in this manner,dna clamping ensures and secures a successful detection for dna sequence homology. the clamp is characterized by a structural change of redβ and a remarkable stability against force up to 200 pn. our findings not only present a detailed explanation for ssap action but also identify the dna clamp as a very stable,noncovalent,dna–protein interaction. © 2015 ander et al.