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Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains
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نویسنده
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wang c. ,sang j. ,wang j. ,su m. ,downey j.s. ,wu q. ,wang s. ,cai y. ,xu x. ,wu j. ,senadheera d.b. ,cvitkovitch d.g. ,chen l. ,goodman s.d. ,han a.
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منبع
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plos biology - 2013 - دوره : 11 - شماره : 2
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چکیده
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Two-component systems (tcss) are important for the adaptation and survival of bacteria and fungi under stress conditions. a tcs is often composed of a membrane-bound sensor histidine kinase (sk) and a response regulator (rr),which are relayed through sequential phosphorylation steps. however,the mechanism for how an sk is switched on in response to environmental stimuli remains obscure. here,we report the crystal structure of a complete cytoplasmic portion of an sk,vick from streptococcus mutans. the overall structure of vick is a long-rod dimer that anchors four connected domains: hamp,per-arnt-sim (pas),dhp,and catalytic and atp binding domain (ca). the hamp,a signal transducer,and the pas domain,major sensor,adopt canonical folds with dyad symmetry. in contrast,the dimer of the dhp and ca domains is asymmetric because of different helical bends in the dhp domain and spatial positions of the ca domains. moreover,a conserved proline,which is adjacent to the phosphoryl acceptor histidine,contributes to helical bending,which is essential for the autokinase and phosphatase activities. together,the elegant architecture of vick with a signal transducer and sensor domain suggests a model where dhp helical bending and a ca swing movement are likely coordinated for autokinase activation. © 2013 wang et al.
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آدرس
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state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen,china,department of molecular and computational biology,university of southern california,los angeles,ca, United States, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China, department of biology and technology,tsinghua university,beijing, China, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China, division of biomedical science,herman ostrow school of dentistry of university of southern california,los angeles,ca, United States, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China, dental research institute,faculty of dentistry,university of toronto,toronto,on, Canada, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China, dental research institute,faculty of dentistry,university of toronto,toronto,on, Canada, dental research institute,faculty of dentistry,university of toronto,toronto,on, Canada, department of molecular and computational biology,university of southern california,los angeles,ca, United States, division of biomedical science,herman ostrow school of dentistry of university of southern california,los angeles,ca, United States, state key laboratory for cellular stress biology,school of life sciences,xiamen university,xiangan,xiamen, China
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Authors
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