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Polymorphic cis- and Trans-Regulation of human gene expression
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نویسنده
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cheung v.g. ,nayak r.r. ,wang i.x. ,elwyn s. ,cousins s.m. ,morley m. ,spielman r.s.
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منبع
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plos biology - 2010 - دوره : 8 - شماره : 9
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چکیده
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Expression levels of human genes vary extensively among individuals. this variation facilitates analyses of expression levels as quantitative phenotypes in genetic studies where the entire genome can be scanned for regulators without prior knowledge of the regulatory mechanisms,thus enabling the identification of unknown regulatory relationships. here,we carried out such genetic analyses with a large sample size and identified cis- and trans-acting polymorphic regulators for about 1,000 human genes. we validated the cis-acting regulators by demonstrating differential allelic expression with sequencing of transcriptomes (rna-seq) and the trans-regulators by gene knockdown,metabolic assays,and chromosome conformation capture analysis. the majority of the regulators act in trans to the target (regulated) genes. most of these trans-regulators were not known to play a role in gene expression regulation. the identification of these regulators enabled the characterization of polymorphic regulation of human gene expression at a resolution that was unattainable in the past. © 2010 cheung et al.
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آدرس
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howard hughes medical institute,philadelphia,pa,united states,department of pediatrics,university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa,united states,department of genetics,university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa,united states,university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa, United States, medical scientist training program,university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa, United States, howard hughes medical institute,philadelphia,pa, United States, university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa, United States, university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa, United States, university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa, United States, department of genetics,university of pennsylvania,the children's hospital of philadelphia,philadelphia,pa, United States
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Authors
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