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The DNA methylome of human peripheral blood mononuclear cells
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نویسنده
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li y. ,zhu j. ,tian g. ,li n. ,li q. ,ye m. ,zheng h. ,yu j. ,wu h. ,sun j. ,zhang h. ,chen q. ,luo r. ,chen m. ,he y. ,jin x. ,zhang q. ,yu c. ,zhou g. ,sun j. ,huang y. ,zheng h. ,cao h. ,zhou x. ,guo s. ,hu x. ,li x. ,kristiansen k. ,bolund l. ,xu j. ,wang w. ,yang h. ,wang j. ,li r. ,beck s. ,wang j. ,zhang x.
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منبع
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plos biology - 2010 - دوره : 8 - شماره : 11
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چکیده
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Dna methylation plays an important role in biological processes in human health and disease. recent technological advances allow unbiased whole-genome dna methylation (methylome) analysis to be carried out on human cells. using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand),we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (pbmc) from the same asian individual whose genome was deciphered in the yh project. pbmc constitute an important source for clinical blood tests world-wide. we found that 68.4% of cpg sites and < 0.2% of non-cpg sites were methylated,demonstrating that non-cpg cytosine methylation is minor in human pbmc. analysis of the pbmc methylome revealed a rich epigenomic landscape for 20 distinct genomic features,including regulatory,protein-coding,non-coding,rna-coding,and repeat sequences. integration of our methylome data with the yh genome sequence enabled a first comprehensive assessment of allele-specific methylation (asm) between the two haploid methylomes of any individual and allowed the identification of 599 haploid differentially methylated regions (hdmrs) covering 287 genes. of these,76 genes had hdmrs within 2 kb of their transcriptional start sites of which > 80% displayed allele-specific expression (ase). these data demonstrate that asm is a recurrent phenomenon and is highly correlated with ase in human pbmcs. together with recently reported similar studies,our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies. © 2010 li et al.
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آدرس
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bgi-shenzhen,shenzhen,guangdong, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai,china,epigentic laboratory,bio-x center,shanghai jiaotong university,shanghai, China, bgi-shenzhen,shenzhen,guangdong,china,beijing institute of genomics,chinese academy of sciences,beijing,china,the graduate university of chinese academy of sciences,beijing, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong,china,school of bioscience and biotechnology,south china university of technology,guangzhou, China, bgi-shenzhen,shenzhen,guangdong, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai, China, bgi-shenzhen,shenzhen,guangdong,china,school of bioscience and biotechnology,south china university of technology,guangzhou, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai, China, cancer epigenetics and gene therapy program,shanghai cancer institute,shanghai jiaotong university,shanghai, China, bgi-shenzhen,shenzhen,guangdong, China, kunming institute of zoology,chinese academy of sciences,yunnan, China, department of biology,university of copenhagen,copenhagen, Denmark, bgi-shenzhen,shenzhen,guangdong,china,institute of human genetics,university of aarhus,aarhus, Denmark, institute of genetics and developmental biology,chinese academy of human sciences,beijing, China, kunming institute of zoology,chinese academy of sciences,yunnan, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, bgi-shenzhen,shenzhen,guangdong, China, ucl cancer institute,university college london,london, United Kingdom, bgi-shenzhen,shenzhen,guangdong,china,department of biology,university of copenhagen,copenhagen, Denmark, bgi-shenzhen,shenzhen,guangdong, China
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Authors
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