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A genetically encoded tag for correlated light and electron microscopy of intact cells,tissues,and organisms
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نویسنده
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shu x. ,lev-ram v. ,deerinck t.j. ,qi y. ,ramko e.b. ,davidson m.w. ,jin y. ,ellisman m.h. ,tsien r.y.
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منبع
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plos biology - 2011 - دوره : 9 - شماره : 4
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چکیده
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Electron microscopy (em) achieves the highest spatial resolution in protein localization,but specific protein em labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. here we introduce minisog (for mini singlet oxygen generator),a fluorescent flavoprotein engineered from arabidopsis phototropin 2. minisog contains 106 amino acids,less than half the size of green fluorescent protein. illumination of minisog generates sufficient singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product resolvable by em. minisog fusions to many well-characterized proteins localize correctly in mammalian cells,intact nematodes,and rodents,enabling correlated fluorescence and em from large volumes of tissue after strong aldehyde fixation,without the need for exogenous ligands,probes,or destructive permeabilizing detergents. minisog permits high quality ultrastructural preservation and 3-dimensional protein localization via electron tomography or serial section block face scanning electron microscopy. em shows that minisog-tagged syncam1 is presynaptic in cultured cortical neurons,whereas minisog-tagged syncam2 is postsynaptic in culture and in intact mice. thus syncam1 and syncam2 could be heterophilic partners. minisog may do for em what green fluorescent protein did for fluorescence microscopy. © 2011 shu et al.
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آدرس
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howard hughes medical institute,university of california at san diego,la jolla,ca,united states,department of pharmacology,university of california at san diego,la jolla,ca, United States, department of pharmacology,university of california at san diego,la jolla,ca, United States, national center for microscopy and imaging research,center for research on biological systems,university of california at san diego,la jolla,ca, United States, howard hughes medical institute,university of california at san diego,la jolla,ca,united states,division of biological science,section of neurobiology,university of california at san diego,la jolla,ca, United States, national high magnetic field laboratory and department of biological science,the florida state university,tallahassee,fl, United States, national high magnetic field laboratory and department of biological science,the florida state university,tallahassee,fl, United States, howard hughes medical institute,university of california at san diego,la jolla,ca,united states,division of biological science,section of neurobiology,university of california at san diego,la jolla,ca, United States, national center for microscopy and imaging research,center for research on biological systems,university of california at san diego,la jolla,ca,united states,department of neurosciences,university of california at san diego,la jolla,ca, United States, howard hughes medical institute,university of california at san diego,la jolla,ca,united states,department of pharmacology,university of california at san diego,la jolla,ca,united states,department of chemistry and biochemistry,university of california at san diego,la jolla,ca, United States
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Authors
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