Kappa Casein Gene Polymorphism in Holstein Chinese Cattle

Kappa casein gene polymorphism has received a considerable attention because of its correlation with milk quality, composition and technological properties. the polymorphism of kappa casein gene (k-cn) was detected in holstein chinese cattle. a 218 bp sequence in exon iv of 319 holstein chinese cattle blood samples were amplified using polymerase chain reaction-single strand conformation (pcrsscp) technique. sequence analysis revealed one single nucleotide polymorphism (snp) t/c snp in exon 1v at nucleotides (80), moreover; three genotypes tt, tc and cc were also identified with following frequencies: 0.40, 0.34 and 0.26%, respectively. the allele frequency for t and c found to be 0.6 and 0.4 %, respectively. allele frequencies in the population fitted with hardy–weinberg equilibrium (p>0.05). analysis of genetic polymorphism of k-casein at exon 1v exhibited medium polymorphism information content (pic=0.36).