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Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR
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نویسنده
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Yang Xiaohong ,Hou Lidan ,Ye Jing ,He Qigai ,Cao Shengbo
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منبع
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pakistan veterinary journal - 2012 - دوره : 32 - شماره : 1 - صفحه:134 -135
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چکیده
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To investigate, whether mosquitoes could be potential vectors that maintain or transmit pcv2, 59 mosquito samples were collected from suspicious pcv2-infected pig farms in hubei and anhui province, china. total dna from these mosquitoes was extracted and then tested for presence of pcv2 nucleic acid by pcr. four (6.78%) samples showed the positive result. subsequently, the positive pcr product was cloned into peasy-t1 vector and sequenced. sequence analysis displayed that homology between the pcr products and pcv2 strains were more than 94%. these results demonstrate that pcv2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as mechanical transmission vectors of pcv2.
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کلیدواژه
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Mosquitoes ,PCV2 ,Polymerase chain reaction ,(PCR) ,Vector
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آدرس
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Huazhong Agricultural University, College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China, Huazhong Agricultural University, College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China, Huazhong Agricultural University, College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China, Huazhong Agricultural University, College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China, Huazhong Agricultural University, College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China
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Authors
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