Optimization of quality control factors for indigenous mycoplasma gallisepticum bacterin preparation and their impact on immunoprophylaxis in broilers

To inactivate locally isolated mycoplasma gallisepticum (mg),phenol,formaldehyde and binary ethylenimine were optimized for their concentrations and exposure time. their effect on immunoprophylaxis induction was then assessed by using different levels of immunogen/pcv and different adjuvants. 0.10m,0.125% and 0.6% concentrations of binary ethylenimine,formaldehyde and phenol inactivated mg colonies within 24,8 and 4 hours respectively. formaldehyde inactivated vaccine with 1.5% immunogen/pcv and montanide oil as adjuvant induced significantly (p<0.05) higher anti mg antibody titer than other locally prepared vaccines. conclusion is that montanide oil adjuvated indigenous formalized bacterin can induce desired immune response. © 2015 pvj.