Comparison of three rapid tests for the detection of β-lactamase production among bacterial isolates

The current study compares the performance and evaluated the practicality of three rapid detection methods for β-lactamase production among 48 veterinary bacterial isolates (16 s. aureus,18 e. coli,and 14 s. typhimurium) in korea. antimicrobial susceptibility was determined by broth microdilution method and β-lactamase production was detected by iodometric,acidimetric,and chromogenic methods. seventy five percent of s. aureus,44% of e. coli,and 35% of s. typhimurium were resistant to penicillin,ampicillin,and amoxicillin,respectively. overall,the iodometric test yielded the highest positive results (91.67%),followed by chromogenic test (66.67%) and acidimetric test (47.92%). the sensitivities and specificities of the three different tests varied for s. aureus,e. coli,and s. typhimurium isolates. our results showed that these test methods may applicable to monitor β-lactamase production among various microorganisms and to predict β-lactam antibiotic resistance. © 2016 pvj.