Maggot antimicrobial peptide effect on TGF-β4 and TNF-α mRNA expression in small intestinal mucosa from Salmonella pullorum-Infected chickens

To explore the effects of maggot antimicrobial peptide treatment of salmonella pullorum-infected chickens on the mrna expression levels of tgf-β4 and tnf-α in small intestinal mucosa,the duodenum,jejunum and ileum mucosa were isolated 3,5 and 7 days after induced amp,non-induced amp,antibiotics or maggot treatment and the tgf-β4 and tnf-α mrna expression was evaluated by quantitative real-time pcr. we found that mrna of tgf-β4 was highly expressed in the duodenum,jejunum,and ileum when salmonella pullorum-infected chickens were treated with antibiotics,non-induced amp,and amp,respectively (p < 0.001). similarly,tnf-α mrna was highly expressed in the duodenum and jejunum when salmonella pullorum-infected chickens were treated with induced amp and maggots,respectively (p < 0.001),and the expression of tnf-α mrna was higher in response to the use of induced amp than antibiotics on day 5 after treatment (p < 0.05). therefore,maggot amp functional efficiency,along with evaluation of tgf-β4 and tnf-α produced by small intestinal mucosa,may be useful for the prevention and treatment of salmonella pullorum when developing new chicken feed additives. © 2017 pvj.