Effect of kuqin compound total polysaccharides on CD4+,CD8+ T cells and cytokines of blood in dogs infected by canine parvovirus

With the purpose of researching on the effect of compound kuqin total polysaccharides on t cell subsets and cytokines of blood in canines with canine parvovirus,the model was established,and 120 canines were separated into 6 groups,such as blank control group (group i),model group (group ii),positive medicine group (group iii),compound kuqin total polysaccharides high dose group (group iv),middle dose group (group v),low dose group (group vi). we set the time point when the virus was injected into canines for 6 hours as day 0,and collected the blood sampling from the canine saphenous vein at different time. finally,we detected the percentages of t lymphocyte subsets and the content of il-2,il-4 and ifn-γ. compared with group i,the percentage of cd3+cd4+ t cells in group ii decreased in 0-35d,cd3+ t cells decreased and cd3+cd8+ t cells increased in 21-35d,the content of il-4 and ifn-γ decreased from day 7 to 21,il-2 decreased from day 21 to 35; compared with group ii,the percentages of cd3+,cd3+cd4+ t cells in group iii and group iv increased in 7-28d,cd3+cd8+ t cells in group iv and v decreased in 7-35d,the content of il-2 in the group iv to vi increased in 14-28d,il-4 and ifn-γ in the group iv to vi increased in 0-35d. the result showed that the high dose compound kuqin total polysaccharides can enhance the secretion of il-2,il-4,ifn-γ and increase the percentages of cd3+,cd3+cd4+ t cells,and decrease the percentage of cd3+cd8+ t cells. based on the results,we concluded that the total polysaccharides of compound kuqin can regulate canine immune functions and improve the antiviral ability in parvovirus infection. © 2017 pvj.