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Clonal expansion of sulfonamide resistant Escherichia coli isolates recovered from diarrheic calves
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نویسنده
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adnan m. ,khan h. ,kashif j. ,ahmad s. ,gohar a. ,ali a. ,khan m.a. ,shah s.s.a. ,hassan m.f. ,irshad m. ,khan n.a. ,rahman s.u.
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منبع
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pakistan veterinary journal - 2017 - دوره : 37 - شماره : 2 - صفحه:230 -232
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چکیده
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A total of 30 escherichia coli isolates from 50 random samples of diarrheic calves were screened against resistance to sulfonamides. minimum inhibitory concentration (mic) by microdilution method indicated that 25/30 (83.3%) were found resistant to sulfadimidine,27/30 (90%) were resistant to sulfadiazine,while 22/30 (73.3%) were found resistant to sulfamethaxole. these phenotypically sulfonamide resistant isolates were then probed for the presence of sul genes (sul-1-3) by polymerase chain reaction (pcr) using specific primers. pcr results revealed that 23/25,25/27 and 20/22 isolates carried sul-1 gene,respectively. interestingly,all these isolates were found negative for the presence of sul-2 and sul-3 genes. of note,no sul genes could be verified in 02 phenotypically resistant isolates. the abundance of sul1 gene and absence of sul-2 and sul-3 genes indicating a clonal expansion of sulfonamide resistant e. coli that might be linked to excessive abuse of sulfonamides in animals. © 2017 pvj.
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کلیدواژه
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Diarrhea; E. Coli; Minimum inhibitory concentration; Sul genes; Sulfadimidine resistance
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آدرس
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department of pharmacology,sindh agriculture university tando jam, Pakistan, department of animal health,the university of agriculture peshawar, Pakistan, department of pharmacology,sindh agriculture university tando jam, Pakistan, department of animal health,the university of agriculture peshawar, Pakistan, college of veterinary sciences and ah,abdul wali khan university,mardan, Pakistan, centre for human genetics,hazara university,mansehra, Pakistan, department of pharmacology,sindh agriculture university tando jam, Pakistan, department of animal health,the university of agriculture peshawar, Pakistan, shaheed benazir bhutto university of veterinary and animal sciences,sakrand sindh, Pakistan, department of preventive dentistry,aljouf university, Saudi Arabia, department of nutrition,the university of agriculture,peshawar, Pakistan, college of veterinary sciences and ah,abdul wali khan university,mardan, Pakistan
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Authors
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