Optimising carbon and nitrogen sources for L-Glutamic acid production by brevibacterium strain NIAB SS-67

Glutamic acid has attracted attention for its pharmaceutical and food applications. there is a 13.3% annual market growth and needs extensive research work on its over-production using new organisms or innovative technology. to increase glutamate yield and productivity,we used brevibacterium strain niab ss-67 and got 38 g glutamic acid/l of glucose-trypticase (l-6) medium (ph 7.0) at 30 °c further enhancement in glutamate production was sought by optimising c and n sources using research techniques. out of nine c sources (glucose galactose dextrin,lactose,maltose,sorbose,xylose,fructose,sucrose) and six n sources (urea,ammonium chloride,ammonium sulphate,ammonium nitrate,ammonium phosphate,potassium nitrate and sodium nitrate) tested,12% dextrin and 2% ammonium sulphate revealed the best results,yielding up to 60.8 g glutamic acid /l (50% enhancement) after 48 h fermentation. product yield (0.23 g/g on glucose medium) increased to 0.61 g/g with glutamic acid volumetric productivity of 2.1 g/l h,which is 1.75-fold higher than that reported on other brevibacterium strains,grown under identical growth conditions copyright 2011 zoological society of pakistan.