Quantitative profiling of serum protein fractions in young obese human

Study concerns the investigation of electrophoretically separated serum protein profile of obese (n=18) and control persons (n=9) with ages ranging 20-40 years. sodium dodecyl sulphate polyacrylamide gel electrophoreses (sds-page) was performed to study the serum protein fractions. the data was quantified by gene genius bio-imaging gel documentation system and analyzed statistically using student't' test to find out the difference of particular protein fractions in obese subjects when compared to controls. fourteen protein fractions were detected ranging between 152-15 kda. protein fractions of 114,34,30,and 17 kda did not vary considerably. significant elevation in 38,26 and 20 kda protein fractions,whereas,significant reduction in 152,131,76,66,49 and 43 kda protein fractions was observed. protein fraction of 15 kda,observed in normal subjects,failed to manifest in any of the obese individuals. serum protein profile,suggests that some fraction alterations in obese individuals are strong indicators of their roles in alteration of body mass. copyright 2011 zoological society of pakistan.