RT-PCR evaluation of foot and mouth disease serotype O in Saliva,tracheal and vesicular samples of goats in Punjab,Pakistan

The aim of present study was to evaluate the rt-pcr technique in the original field samples from goat for the diagnosis of foot and mouth disease (fmd) within 24 hours. a total of 280 samples were collected from thirty herds of goats in twelve different outbreaks clinically suspected for fmd near outbreak epicenters of other livestock and evaluated by rt-pcr. with 171 positive results. universal primer pair p1/p2 detected 30% and if/ir detected 61% from total collected samples. au(0)/au(rcv) and au(o)/pnk61 primer pairs confirmed o serotype of fmd in 90.6% and 74.4% respectively out of 43 representative samples,whilst 0-1c(ars4)/ pnk61 o primer pairs detected only 18.6% samples. the vp1 gene sequence analysis revealed o serotype of fmd panasia1 lineage. the maximum samples can be detected by 1f/1r,au(o)/au(rev) and au(o)/pnk61 primer pairs; whilst p1/p2,au(o)/au(rev) and ars4/nk61 can be a better combination for the sequence analysis from original field materials of goats.