Quantitation of metronidazole in pharmaceutical suspension using high performance liquid chromatographic method

A simple and rapid hplc (high performance liquid chromatography) assay procedure has been developed and validated for the quantitative determination of metronidazole benzoate for raw material and commercially available suspensions. the hplc conditions used were,column: c18,(0.5μm particle size,150×4.6mm); detection: uv at 254 nm; injection volume: 20 μl; mobile phase: methanol and de-ionized water (64:40,v/v); isocratic elution under ambient temperature at flow rate of 1.0 ml min -1. the procedure separated metronidazole benzoate,in an overall analysis time of about 10 minutes with metronidazole benzoate eluting at about 7.7 minutes. the injection repeatability was 0.05%,the intraday and interday repeatability were 0.59 and 0.91%,respectively. the procedure provided a linear response over the concentration range 10-1000 μgml -1 (r 2 = 1.0000) with the limits of detection and quantitation 0.0158 and 10 μg ml -1,respectively. the results show no detectable hydrolysis of metronidazole benzoate in 10 hours. copyright 2011 zoological society of pakistan.