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Effect of first intron on ovalbumin promoter activity
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نویسنده
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xu s. ,kaleri h.a. ,liu h.
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منبع
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pakistan journal of zoology - 2011 - دوره : 43 - شماره : 6 - صفحه:1183 -1189
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چکیده
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The present study was designed to investigate effect of first intron on ovalbumin promoter activity using immortalization of oviduct epithelial cells. the oviduct epithelial cells were cultured and then immortalized using pl-ctagneo virus. the pl-ctagneo virus maintained the structural integrity and growth of oviduct epithelial cells for 18 days whereas growth rate declined continuously with aging and cellular death after 7-10 days in controlled culture. the immortalized oviduct epithelial cells were transfected with pl-ov1345tpagfp and pl-ov2964tpagfp virus,respectively. the dna and mrna abundance of ov1345 virus was 46 and 14 times higher than ov2964 virus. the transcript effect of ov2964 virus was 3-fold higher than ov1345. the result showed that first intron in ov gene has a cis-regulation function for transcription. © 2011 zoological society of pakistan.
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کلیدواژه
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First intron; Immortalization; Ovalbumin promoter activity; Oviduct epithelial cells
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آدرس
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college of animal science and technology,nanjing agricultural university,nanjing,210095,china,college of animal sciences and technology, China, college of animal science and technology,nanjing agricultural university,nanjing,210095,china,department of animal breeding and genetics,faculty of animal husbandry and veterinary sciences, Pakistan, college of animal science and technology,nanjing agricultural university,nanjing, China
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Authors
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