Peripheral administration of the human kisspeptin-10 and 26RFamide inhibits plasma testosterone levels in the adult male broiler breeder birds (Gallus domesticus)

Kisspeptin (kp),a family (belonging to the rfamide superfamily of peptides) of related-peptide hormones encoded by the kiss1 gene,is recently identified as the major player in the neuroendocrine regulation of the mammalian reproduction. despite the well established role of kp in mammals not much is known about their action in non-mammalian species especially in the avian reproductive physiology. therefore,the present study was designed to examine the effect of peripheral administration of human kisspeptin-10 (kp10) on the plasma testosterone (t) concentration in the broiler breeder birds. three different doses (11.5,23,38-nmol; intravenous injection) of kp10 were tested in the intact adult male broiler breeder birds under normal fed conditions. in addition,hcg (30iu) and vehicle (0.5 ml) were administered for control purposes. a single dose (38-nmol) of human 26rfamide (26rfa; another member of rfamide superfamily) was also tested. kp10 administration dose-dependently inhibited (p<0.05-0.005) plasma t levels. likewise,26rfa administration also significantly decreased (p<0.05) plasma t concentration. vehicle administration did not alter plasma t levels. hcg administration stimulated (p<0.01) plasma t levels. thus,the present study demonstrated that human kp10 and 26rfa administration suppressed plasma t levels in the adult male broiler breeder birds. our findings of the present study,therefore,assign a novel role to kp10 and 26rfa,as inhibitor of plasma t secretion in the broiler breeder birds. however,the mechanisms (receptor and intracellular signaling) by which kp10 and 26rfa suppressed the plasma t level in the broiler breeder birds are currently unknown. © 2012 zoological society of pakistan.