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Comparison of methods for DNA extraction from a single chironomid for PCR analysis
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نویسنده
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wang q. ,wang x.
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منبع
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pakistan journal of zoology - 2012 - دوره : 44 - شماره : 2 - صفحه:421 -426
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چکیده
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Genomic dna of single chironomid midges was extracted using the cetyl-trimethyl ammonium bromide (ctab) method,the proteinase k method and the chelex-100 method,respectively. the results suggested that all three methods are effective in extraction of genomic dna of small insects. but comparatively,the quality of dna sample isolated using the ctab method was better than those extracted using the other two methods,if the specimens were stored in 75% ethanol for a long time. however,if the specimens were fresh or stored in 95% ethanol,the chelex-100 and proteinase k methods,using less chemicals hazardous to health,are more rapid than the ctab method. copyright 2012 zoological society of pakistan.
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کلیدواژه
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Chelex-100; Chironomidae; CTAB; DNA-extraction; PCR amplification; Proteinase K
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آدرس
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college of life sciences,nankai university,300071 tianjin,china,tianjin key laboratory of aqua-ecology and aquaculture,department of fisheries science,tianjin agricultural university,300384, China, college of life sciences,nankai university, China
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Authors
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