Dot-ELISA for Newcastle disease,Infectious bursal disease and mycoplasmosis

Newcastle disease (nd),infectious bursal disease (ibd) and mycoplasmosis are common respiratory diseases of poultry affecting several billion rupees business in pakistan. prevalence of these diseases demands specific strategies to control their occurrence. efforts were made to develop dot-elisa for detection of antibodies against ndv,ibd and mycoplasma gallisepticum (mg). dot-elisa was performed on the sera samples collected from sick birds showing symptoms of nd,ibd and mycoplasmosis. antibodies were detected by dot-elisa against ndv,ibdv and mg. study showed that birds were highly exposed to mg in our region. antibodies were detected against ndv,ibdv and mg in 37.2%,32.1% and 54.7% infected birds,respectively. present study also showed that the birds possessed antibodies against more than one pathogen tested: 5.1% of the infected birds possessed antibodies against ndv and ibdv; 9.5% against ndv and mg; and 11.7% against ibdv and mg. data also showed that 2.9% of the infected birds were exposed to all the three pathogens that were tested. antibodies were also detected in the sera obtained from healthy birds against all these three pathogens. around 9.5%,8.4% and 15.5% sera were found to contain antibodies against ndv,ibdv and mg,respectively. present study also showed that the prevalence of co-infections are 1.9% (ndv and ibdv),3% (ndv and mg) and 3.3% (ibdv and mg),respectively. dot-elisa using specific antigens from pathogens is very specific,highly sensitive,less time consuming with little false result. a diagnostic kit can be prepared for large number of pathogens; this combine approach of several antigens on a single strip can play an important role in the diagnosis of poultry disease at early stage,and help in controlling infectious diseases in poultry sector in pakistan. copyright 2012 zoological society of pakistan.