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Rapid species identification of morphologically similar cetacean species kogia sima and k. breviceps by high-resolution melt analysis
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نویسنده
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wang y.-t. ,chou l.-s. ,lo c. ,yang w.-c.
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منبع
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pakistan journal of zoology - 2013 - دوره : 45 - شماره : 1 - صفحه:273 -277
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چکیده
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Due to their morphological similarities,the differentiation of pygmy (kogia breviceps) and dwarf (k. sima) sperm whales is problematic. here we report a quick and efficient method for distinguishing the two species based on differences in high-resolution melt (hrm) curves of amplicons from the mitochondrial dna control region. k. sima and k. breviceps generated distinct melt curve profiles as molecular fingerprints for species typing. the mitochondrial identities of the 38 samples were all correctly typed using hrm analysis. it is estimated that a 30-sample identification work of kogia spp. can be accomplished in 2-3 h using the hrm pcr assay without regular full sequencing process. the assay substantially simplifies the required work of definitive species assignment before stranding data are used in distributional and ecological studies. copyright 2013 zoological society of pakistan.
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کلیدواژه
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Cetacean; HRM; Kogia; Mitochondrial DNA control region; Species identification
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آدرس
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department of veterinary medicine,national chiayi university,no.580,xinmin rd., Taiwan, institute of ecology and evolutionary biology,national taiwan university,roosevelt rd., Taiwan, department of veterinary medicine,national chiayi university,no.580,xinmin rd., Taiwan, department of veterinary medicine,national chiayi university,no.580,xinmin rd., Taiwan
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Authors
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