Frequency of kras gene mutations in endometrioid carcinoma of uterus in pakistani population

The aim of this study was to find out the frequency of kras gene mutation in endometrioid carcinoma in pakistani population. formalin fixed paraffin embedded 70 samples of endometrioid carcinoma were collected from shaukat khanum memorial cancer hospital and research centre. dna was isolated,followed by pcr amplification using primers of exon 1 of kras gene. sscp analysis was done to detect mutation,which was confirmed by direct sequence analysis. frequency of kras gene mutation was calculated. statistical analysis was done using spss 15.0 software. frequency of kras gene mutation was found to be 34%. out of these 34% mutant samples,62.5% patients were < 50 years of age. well differentiated histological grade was present in 91.7% of mutant samples. copyright 2013 zoological society of pakistan.