Rabbit skin and polydimethylsiloxane as model membranes to evaluate permeation kinetics from topical formulation

This study aimed at evaluating influence of turpentine oil (to) on percutaneous absorption of diclofenac diethylamine (dda),a potent non-steroidal anti-inflammatory agent,using franz-type diffusion cell across two model membranes,namely rabbit skin and polydimethylsiloxane membrane. the flux of dda across the rabbit skin and polydimethylsiloxane membrane using different ratios (1%,2%,3% and 4%) of turpentine oil progressively increased with increasing concentration of turpentine oil content with the formulation containing 4% enhancer showed maximum flux of 40.06 (μg/cm2/h) and 6.19 (μg/cm2/h),respectively. the input-rate of active from all solutions has shown a trend of increase with the increase in the concentration of enhancer in the solutions. the vehicle used was predominantly influencing the diffusion of the drug into the skin rather than the partition coefficient throughout the study. consequently,changes in diffusion and/or partition may occur as a result of quick absorption followed by the depletion of drug in the donor in the presence of turpentine oil inside the skin /or membrane over time which validates our results. copyright 2013 zoological society of pakistan.