|
|
|
|
A protocol for DNA extraction from feces of the wild pygmy lorises,Nycticebus pygmaeus
|
|
|
|
|
|
|
|
نویسنده
|
zhang l. ,cai j. ,xiao c. ,wang z.
|
|
منبع
|
pakistan journal of zoology - 2013 - دوره : 45 - شماره : 5 - صفحه:1235 -1239
|
|
چکیده
|
We developed a new method to isolate dna from wild pygmy lorises' feces. the feces were washed two or three times with precooled acetone,which removed numerous potential pcr inhibitors. the dna was purified with phenol/chloroform after incubation with proteinase k. d-loop region and cytochrome b were amplified and sequenced successfully from the extracted dna. comparison with the pcr products demonstrated the fecal dna extracted by the improved protocol was better than the fecal dna extracted without acetone preprocessing. copyright 2013 zoological society of pakistan.
|
|
کلیدواژه
|
DNA extraction; Fecal DNA; Noninvasive sampling; Nycticebus pygmaeus; Pygmy lorises
|
|
آدرس
|
school of life sciences,yunnan normal university,kunming 650500,china,jiangsu key laboratory for biodiversity and biotechnology,college of life sciences,nanjing normal university, China, school of life sciences,yunnan normal university, China, school of life sciences,yunnan normal university, China, school of life sciences,yunnan normal university, China
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|