Development of microsatellite markers for leptobotia elongata (cypriniformes: Cobitidae) using 454 sequencing and cross-species amplification

Thirty microsatellite markers were developed from leptobotia elongata through 454 sequencing and 27 were polymorphic among 24 individuals,with an average of 9.67 alleles per locus,and the mean observed and expected heterozygosities were 0.81 and 0.79,respectively. polymorphism information content (pic) ranged from 0.416 to 0.933. in addition,cross-species amplification was tested for all 30 loci in four other loaches of cobitidae (leptobotia microphthalma,botia superciliaris),and nemacheilidae (oreias dabryi,triplophysa stenura). the results showed a significant high level of transferability (63%-97%),which means the possibility of these markers applying to other related species. the microsatellite loci described here would be useful in conservation of l. elongata and possibly some other species. copyright 2014 zoological society of pakistan.