Adaptation of WM-68 hybridoma cell-line in minimal serum and serum free culture conditions

Wm-68 cell line has been shown to produce anti-cd48 antibody - highly specific to b cell malignancies. for their clinical usage,cultivation of wm68 cells in animal serum pose a technical and safety disadvantage. therefore,the present study was aimed to establish an adaptation protocol for wm68 hybridoma cell line - from high to a low serum concentration and finally into a serum free culture condition. the wm68 hybridoma cell line cultured in 10% fbs exhibited excellent cell culture profiles - cell viability,density and antibody production. similar trend was observed when wm68 cell line was cultured in 0.5% fbs. however,in 0.5% fbs,data revealed poor antibody production in the stationary phase compared to 10% fbs. finally,wm68 cell line cultured in 0.5% fbs,was passaged and plated in a serum free condition. unfortunately,cells didn't survive past day one and manifested obvious signs of apoptosis. taken together,these data suggested that 10% fbs and 0.5% fbs are suitable culture conditions for the propagation of wm68 hybridoma cell line and to generate antibodies. thus,employing additional media factors and avant-garde cell culture techniques can enhance our understanding of the wm68 cell line culture requirements in a serum free media. copyright 2014 zoological society of pakistan.