Effect of different synthetic hormones and/or their analogues on induced spawning in Channa marulius

Effect of different synthetic hormones and/or their analogues have been evaluated on breeding performance of channa marulius using four different hormonal treatments. males were injected with various combinations of ovaprim (gonadotropin releasing and dopamine antagonist) with human menopausal gonadotropin (hmg),human chorionic gonadotropin (hcg) and hcg+hmg; whereas,females received combination of ovaprim with hmg and hcg. after 12,16,20 and 24 h of first dose,injection of ovaprim were administered and blood testosterone,follicle stimulating hormone and luteinizing hormone were analyzed every 4 h for 48 h. results indicated that combination of hcg+hmg and ovaprim+hcg were effective and reliable synthetic hormones for induced spawning in c. marulius. fecundity and egg fertilization rate was highest for hcg+hmg at latency period of 43.20-44.45 h. blood hormonal levels were non-significant (p<0.05) and increased gradually till 28 h of post injection and then decreased gradually. fish injected with ovaprim+hcg does not spawn at all. eggs obtained were yellow,spherical,non-adhesive and translucent. after fertilization,first cleavage was observed within 2 h,second was between 3-4 h and after 4-6 h,a shield appeared inside and two-layered structure appeared with an outer epiblast and inner hypoblast. however,no further development was observed as the eggs succumb to fungal infection. copyright 2015 zoological society of pakistan.