Lack of association between apolipoprotein E polymorphism and acute myocardial infarction in a Pakistani population

The aim of this study was to examine the association of apolipoprotein e(apoe) polymorphism with acute myocardial infarction (ami) in a pakistani population. a cross-sentional study involving 218 ami patients (166 males and 51 females; age 30-75 years and 171 healthy controls (121 males and 50 females,age 19-76 years) was carried out on a hospital-based pakistani population. fasting serum concentrations of total cholesterol,low density lipoprotein (ldl)-cholesterol,high density lipoprotein (hdl)-cholesterol,triglycerides and glucose were determined using kit methods. dna was extracted and apoe polymorphism was studied by polymerase chain reaction (pcr) followed by restriction fragment length polymorphism (rflp). apo e3/e3 genotype was the most common genotype in both cases and controls (78% and 74%,respectively),while e2/e3 genotype was the least common one in this cohort (6.5% and 7.97%,respectively). frequencies of e2,e3 and e4 alleles in ami patients (0.078,0.766 and 0.155,respectively) were not significantly different from the frequencies of these alleles in healthy controls (0.11,0.708,0.18,respectively; x2=1.95,p value 0.37). moreover,no association was observed between genotypes - e3/e4,e2/e3,e3/e3 and serum lipids in ami patients. apoe polymorphism was not found to be associated with ami in a hospitalbased population in karachi. copyright © 2015 zoological society of pakistan.