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Hyperproduction of alginate by mutated strain of azotobacter vinelandii through submerged fermentation
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نویسنده
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saeed s. ,hashmi a.s. ,ikram-ul-haq institute of industrial biotechnology ,tayyab m. ,awan a.r. ,anjum a.a. ,firyal s.
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منبع
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pakistan journal of zoology - 2016 - دوره : 48 - شماره : 5 - صفحه:1585 -1589
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چکیده
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The present study was designed to improve the parent strain of azotobacter vinelandii nrrl-14641 for hyper-production of alginate by random mutagenesis. the mutant (etbr-02) obtained after ethidium bromide treatment gave 2.11 fold higher alginate production than the parent strain. the maximum concentration of alginate (15.61 g/l) was obtained by utilizing 10% (w/v) wheat bran,8% (v/v) inoculum size at 48 h of incubation period,ph 7.0,30°c and agitation speed of 200 rpm. inclusion of 2.5% corn steep liquor raised the alginate concentration to 15.8 g/l. these optimized conditions can be executed for the mass production of alginic acid on pilot scale. © copyright 2016 zoological society of pakistan.
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کلیدواژه
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Azotobacter vinelandii; Hyper-production of alginate; Mutated strain
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آدرس
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institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, government college university,lahore, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, department of microbiology,university of veterinary and animal sciences,lahore, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan
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Authors
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saeed ,hashmi ,ikram-ul-haq ,tayyab ,awan ,anjum ,firyal
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