The expression and role of caveolin-1 in different development periods of drosophila melanogaster

Caveolin-1 is an iconic structural molecule of caveolae. because of its pivotal role in the process of cell proliferation,apoptosis and other related signaling pathways of human tumors,caveolin-1 has been extensively studied. it has been found that caveolin-1 expresses in various types of invertebrates and vertebrates and is highly conserved in evolution. however,there has been no report about the caveolin-1 of insect so far. in the present study,caveolin-1 was amplified in the larvae and adults of drosophila melanogaster to observe their expression levels. the in vivo distributions of caveolin-1 were observed in larvae,pupae and adults through immunofluorescence and confocal technology. furthermore,the expression of caveolin-1 was detected in larvae,pupae and adults through western blotting. the results showed that caveolin-1 exists in d. melanogaster,and its expression and distribution varied in different developmental stages. the in vivo distribution of caveolin-1 was mainly in the brain,muscles and gut wall in adult,a little in the brain and fat bodies in pupa and a little in the fat bodies in larva. the expression levels of caveolin-1 were significantly higher than in adults in the larvae and pupae. the expression of the protein was significantly higher in the female adult than in the adult male. © copyright 2016 zoological society of pakistan.