An novel SNP in LPL gene exon 8 associated with meat quality traits in ducks

Lipoprotein lipase (lpl) plays a crucial role in the metabolism and transport of lipids. the polymorphism of the lpl gene exon 8 and associations with duck meat quality traits were investigated using pcr-sscp analysis and dna sequencing. a novel silent mutation 1251 t>c was first identified located in exon 8 of the lpl gene. two alleles (t and c) and three genotypes designed as tt,tc and cc were detected in the pcr products. the t allele and tt genotype were dominant in the five breeds. the 1251 t>c locus belonged to moderately informative polymorphism except for the yinjiang ducks. the genotype distribution of the xingyi,jinding and cherry valley populations deviated from hardy-weinberg equilibrium but not the sansui and yinjiang populations. the 1251 t>c locus was significantly associated with meat quality traits except for ph,water holding capacity,intramuscular fat,c14:1,and c15:0. significant additive effects were detected for c12:0,c14:0,c16:0,c16:1,c17:0,c18:2,c20:1,c20:3,pufa and efa,significant dominance effects for shear value,c14:0,c17:0,c18:0,c18:1,c18:3,c20:0,c20:1,c20:2,c20:4 and ufa. the results suggested that the 1251c>g polymorphism might be useful genetic markers in duck breeding to improve the content of beneficial fatty acids. © copyright 2016 zoological society of pakistan.