Filter paper activity producing potential of aeromonas species isolated from the gut of labeo rohita

In this study different species of aeromonas were isolated from gut of labeo rhoita and identified by 16srdna gene sequencing technology. all isolated species were evaluated for exoglucanase production in submerged fermentation. of all tested species,aeromonas bestiarum was found best for maximum production of exoglucanase after 24 h of fermentation at 35 °c using sugarcane bagasse as substrate. to enhance exoglucanase production,various factors were screened by plackettt-burman designe and optimizations of significant parameters were carried out by boxbehnken design of response surface methodology. among nine parameters screened,substrate concentration,yeast extract and (nh4)2so4 concentration were found significant. the optimized levels of these parameters were. 2.5% sugarcane bagasse,0.2% yeast extract and 0.6% (nh4)2so4 which yielded maximum (3.766 iu) exoglucanase production after 24h of fermentation period. these results suggested the potential utilization of this strain for nutritional purpose to promote the growth of fishes for commercialization of aquaculture. © copyright 2016 zoological society of pakistan.