Larvicidal activity of N1,N1,N4,N4-tetramethylpiperazine-1,4-dicarboxamide against Aedes caspius and Culex pipiens (Diptera: Culicidae)

The present work describe a safe,mild,eco-friendly and versatile method for the synthesis of n1,n1,n4,n4-tetramethylpiperazine-1,4-dicarboxamide. the prepared compound was screened against aedes caspius (pallas) and culex pipiens (linnaeus) larvae and compared to its parent piperazine moiety. likewise,histological structure of gut of treated third instar larva was considerably disturbed. the results demonstrated that substituted urea (1,4-dicarboxamide) meddled with the development of the mosquitoes,and had its strongest poisonous impact at 200 ppm for 48 h. the third larval instar treated with the 1,4-dicarboxamide indicated cell devastation,vacuolization of epithelial cells and cell dispersing in little locales of the mid-gut. the 1,4-dicarboxamide appear to be eco-friendly larvicide to control ae. caspius and cx. pipiens. copyright 2016 zoological society of pakistan.