Polymorphism analysis of LHβ gene in sheep

In this study,lhβ gene polymorphism was analyzed by the sequencing technique,snp mutation sites of lhβ gene were detected in five sheep breeds including lanzhou large-tailed sheep,small-tailed han sheep,mongolian sheep,large-tailed han sheep and yuxi fat-tailed sheep,in order to further provide reference to investigate the genetic mechanism of reproductive performance in sheep. the results showed that 3 snp sites of lhβ gene were detected in lanzhou large-tailed sheep,small-tailed han sheep,mongolian sheep,large-tailed han sheep and yuxi fat-tailed sheep,they were c551t c/t,g391a g/a and g394a g/a,respectively. c and t allele frequencies in c551t site were 0.594/0.406,0.811/0.189,0.840/0.160,0.927/0.073 and 0.868/0.132,respectively. g and a allele frequencies in g391a site were 1.000/0.000,0.705/0.295,1.000/0.000,0.711/0.289 and 0.780/0.220,respectively. g and a allele frequencies in g394a site were 1.000/0.000,0.668/0.332,1.000/0.000,0.656/0.344 and 0.698/0.302,respectively. the phylogenetic trees of partial sequences were constructed for partial lhβ gene sequences of different species using dnastar software. the results showed that sheep,goat,water buffalo,yak clustered firstly. hedgehog,bat and whale were far from sheep. so they were alone. finally they clustered with sheep. copyright 2016 zoological society of pakistan.