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CDNA cloning and expression of cyclophilin a (LvCypA) in white leg shrimp,litopenaeus vannamei
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نویسنده
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muhammad f. ,zhi-feng z. ,ming-yu s. ,shafi m.
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منبع
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pakistan journal of zoology - 2017 - دوره : 49 - شماره : 3 - صفحه:935 -941
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چکیده
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In present investigation the cyclophilin a (cypa) of white leg shrimp (litopenaeus vannamei) has been cloned by rapid amplification of cdna (race) and anchored pcr method. the full length of the (lvcypa) had 855 bp,containing 495 bp of open reading frame (orf),encoding 164 amino acids with an estimated molecular mass of 17.6 kda. the lvcypa has four α-strands. tissue distribution of the lvcypa after real time analysis revealed that expression is in order of muscle,gill,lymphoid organ and hepatopancrease. copyright 2017 zoological society of pakistan.
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کلیدواژه
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Cloning; Cyclophilin A; Cyclophilin a gene expression; Litopenaeus vannamei
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آدرس
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key laboratory of marine genetics and breeding,ocean university of china,ministry of education,qingdao,china,centre of excellence in marine biology,university of karachi,karachi, Pakistan, key laboratory of marine genetics and breeding,ocean university of china,ministry of education,qingdao, China, key laboratory of marine genetics and breeding,ocean university of china,ministry of education,qingdao, China, lasbela university of agriculture,water and marine sciences,uthal, Pakistan
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Authors
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