Isolation and molecular characterization of medically important yeasts isolated from poultry slaughterhouses and workers

Early identifcation of yeast to the genus and species level is necessary for effective antifungal therapy,and can also facilitate control of infections. a total of 50 isolates were isolated from 60 swab samples collected from two poultry processing plants represented broiler carcasses swabs and worker (hand swabs and nail scrapings). phenotype-based methods for identifying yeast especially candida species are often diffcult,time-consuming and not enough to identify most yeast species. molecular biological techniques provide a useful alternative approach. concerning with genotypic identifcation,polymerase chain reactionrestriction fragment length polymorphism (pcr-rflp) was applied for identifcation of candida species,cryptococcus albidus,saccharomyces,trichosporn,torulopsis and rhodotorula. using universal primers its1-5.8s-its4 amplifed its region. and it yielded a unique pcr size of approximately 376-930 bp. pcr amplicons were digested with enzyme mspi and the generated bans corresponded to the predicted size. © 2017 zoological society of pakistan.