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Characterization of hGFAP-DsRed transgenic guangxi bama mini-pigs and their offspring
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نویسنده
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zhu x. ,nie j. ,quan s. ,xu h. ,yang x. ,lu y. ,lu k. ,lu s.
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منبع
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pakistan journal of zoology - 2017 - دوره : 49 - شماره : 2 - صفحه:509 -519
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چکیده
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Astrocytes,the most abundant cell type in the mammalian central nervous system (cns),perform many important neurobiological functions. although a large number of transgenic mouse models carrying astrocyte-specifc transgene expression of transgene have made signifcant contributions to understanding astrocytic structure and function in vitro and in vivo,evidence suggests that mouse models are limited and sometimes do not fully replicate the complete spectrum of neurological phenotypes seen in human diseases. on the contrary,pigs,especially mini-pigs,show exciting potential for modeling human cns diseases due to similarities in body size,anatomy,life span,cns structure and neurobiology. previously,via the somatic cell nuclear transfer technique,we successfully generated transgenic guangxi bama mini-pigs carrying a fluorescent protein (dsred) reporter gene regulated by the 2.2-kb human glial fbrillary acidic protein promoter (hgfap-dsred). this study characterized transgene expression in such transgenic guangxi bama mini-pigs and their offspring. our fndings indicate that the hgfap promoter contains matching regulatory elements for directing specifc expression in porcine astrocytes. however,the practical application of hgfap-dsred transgenic guangxi bama mini-pigs in neuroscience research requires solving the germline transmission problem of the phenotype. © 2017 zoological society of pakistan.
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کلیدواژه
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Astrocyte; Central nervous system; Germline transmission; Guangxi Bama minipig; Human glial fbrillary acidic protein gene promoter; Transgenic pig model
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آدرس
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state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning,china,reproductive medicine center of guigang city people's hospital,guigang, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China, state key laboratory for conservation and utilization of subtropical agrobioresources,guangxi high education key laboratory for animal reproduction and biotechnology,college of animal science and technology,guangxi university,nanning, China
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Authors
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