Characteristics of structure and nucleotide polymorphism of the American mink (Neovison vison) growth hormone gene

The nucleotide sequence,variation and gene-structure of the american mink (neovison vison) growth hormone gene was analyzed. for this purpose set of 3 amplicons were sequenced,including 5'-utr and all exons and introns. the study involved 389 animals. the sequencing results revealed the presence of 5 exons (10 bp,161 bp,117 bp,162 bp,201 bp) and 4 introns (245 bp,171 bp,176 bp,290 bp),with a total length of 1745 bp. fourteen polymorphic variable sites were identified: 12 snp substitutions,one single nucleotide deletion and one ins/del polymorphism. the assessment of informativeness and conclusiveness of the identified variable sites shows that the highest ability to differentiate the american mink growth hormone gene genotypes has tri-allelic polymorphism g.1219c>g (pic = 0.3039),and three di-allelic polymorphisms - g.616g>c,g.846a>g and g.931c>t (pic>0.2400). the recognized snps enrich the database of single nucleotide polymorphisms for american mink. copyright 2017 zoological society of pakistan.