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Molecular phylogeny and diversity analysis of HOG deer (Axis porcinus) in Pakistan
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نویسنده
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abbas g. ,nadeem a. ,babar m.e. ,hussain t. ,tahir m.s. ,shehzad w. ,iqbal r.z. ,tayyab m. ,javed m.
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منبع
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pakistan journal of zoology - 2017 - دوره : 49 - شماره : 5 - صفحه:1701 -1712
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چکیده
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Many species of mammals have declined within the past two centuries due to human caused disturbances and the unsustainable use of natural resources. molecular methods have an important role in phylogeny and diversity analysis. in pakistan hog deer (axis porcinus) is an important wild species belongs to the family cervidae. the hog deer is one of the least studied species at molecular level in pakistan. the present study was planned to investigate its molecular phylogeny and diversity analysisusing mitochondrial cytochrome b,cytochrome c and d-loop region. samples were collected from different localities. after dna extraction and quantification,amplification of gene was done by polymerase chain reaction. pcr products were sequenced bi-directionally,aligned and single nucleotide polymorphisms were identified. bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. lower genetic diversity was observed. the finding of this research is prerequisite for future research. copyright 2017 zoological society of pakistan.
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کلیدواژه
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Mitochondrial; Pakistani HOG deer; Phylogenetic
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آدرس
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institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, department molecular biology,virtual university of pakistan,lahore, Pakistan, department molecular biology,virtual university of pakistan,lahore, Pakistan, shaheed,benazir,bhutto university of veterinary and animal sciences,sakrand, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, shaheed,benazir,bhutto university of veterinary and animal sciences,sakrand, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan, institute of biochemistry and biotechnology,university of veterinary and animal sciences,lahore, Pakistan
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Authors
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