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New genetic evidence from three keel-backed liza species based on DNA barcoding confirms morphology-based identification
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نویسنده
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liu l. ,panhwar s.k. ,gao t. ,han z. ,li c. ,sun d. ,song n.
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منبع
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pakistan journal of zoology - 2017 - دوره : 49 - شماره : 5 - صفحه:1901 -1907
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چکیده
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Three liza species exhibiting a dorsal keel have been identified based on morphology: liza affinis,liza carinata,and liza klunzingeri. to confirm the validity of the species by molecular methods,we sequenced a fragment of the cytochrome oxidase subunit i (coi) gene of mitochondrial dna of liza affinis and liza klunzingeri from coastal waters of china and pakistan,respectively. sequences of l. carinata were obtained from genbank. a neighbor-joining tree showed the specimens to form a strong monophyletic group. the mean within-species genetic distances for l. affinis,l. carinata,and l. klunzingeri were 0.26%,0.52%,and 0.47%,respectively. the mean genetic distances among l. affinis,l. carinata,and l. klunzingeri ranged from 12.22% to 14.74%,higher than the threshold for species delimitation. the results confirmed the taxonomic validity of the three liza species and verified that the coi gene could provide effective dna barcoding for identification. copyright 2017 zoological society of pakistan.
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کلیدواژه
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DNA barcoding; Liza affinis; Liza carinata; Liza klunzingeri; Morphological identification
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آدرس
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fisheries college,ocean university of china,qingdao, China, center of excellence in marine biology,university of karachi,karachi, Pakistan, fishery college,zhejiang ocean university, China, fishery college,zhejiang ocean university, China, south china sea fisheries research institute,chinese academy of fishery sciences,guangzhou, China, south china sea fisheries research institute,chinese academy of fishery sciences,guangzhou, China, fisheries college,ocean university of china,qingdao, China
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Authors
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