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An extract of Perilla stem inhibits Src homology phosphatase-1 (SHP)-1 and influences insulin signaling
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نویسنده
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peng l. ,lei z. ,xiao-na x. ,deli w. ,jing s. ,yong-sen w. ,zhi w. ,shu x. ,jun-feng m. ,wan-nan l. ,xue-qi f.
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منبع
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pakistan journal of pharmaceutical sciences - 2015 - دوره : 28 - شماره : 2 - صفحه:421 -424
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چکیده
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Protein tyrosine phosphatases (ptps) are enzymes that catalyze protein tyrosine dephosphorylation of which src homology phosphatase-1 (shp-1) is one of the best-validated,a widely distributed intracellular tyrosine phosphatase that contains two sh2 domains. down regulation of shp-1 tyrosine phosphatases was significantly increased sensitivity to insulin in insulin signaling pathway. through in vitro enzymatic reaction kinetics experiment,we found that the extract of perilla stem was a potential inhibitor to δshp-1,the catalytic domain of shp-1 protein tyrosine phosphatase,and its ic50 was 4ug/ml,and was more sensitive towards shp-1than other ptps,which indicated that shp-1 might be a target of the extract of perilla stem. it can strengthened the level of tyrosine phosphorylation of insulin receptor (ir) and extracellular signal-regulated protein kinase (erk) in hepg2 cells,and then activated the insulin signaling pathway through inhibiting the protein phosphorylation of shp-1. these results demonstrated that the extract of perilla stem could play an important role for diabetes treatment through inhibiting the level of shp-1 in insulin signaling pathway.
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کلیدواژه
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Inhibitor; Insulin sensitivity; Perilla stem; SHP-1
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آدرس
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key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, first hospital of jilin university, China, state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China, key laboratory for molecular enzymology and engineering,jilin university,ministry of education,changchun,china,state engineering laboratory of aids vaccine,jilin university, China
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Authors
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