Rhodamine-123: A p-glycoprotein marker complex with sodium lauryl sulfate

Aim of this study was to investigate the role of sodium lauryl sulfate (sls) as p- glycoprotein inhibitor. the everted rat gut sac model was used to study in-vitro mucosal to serosal transport of rhodamine-123 (rho-123). surprisingly,sls decreases the serosal absorption of rho-123 at all investigated concentrations. investigation reveals complex formation between rhodamine-123 and sodium lauryl sulfate. interaction profile of sls & rho-123 was studied at variable sls concentrations. the sls concentration higher than critical micelle concentration (cmc) increases the solubility of rho-123 but could not help in serosal absorption,on the contrary the absorption of rho-123 decreased. rho-123 and sls form pink color complex at sub-cmc. the sls concentrations below cmc decrease the solubility of rho-123. for further studies,rho-123 & sls complex was prepared by using solvent evaporation technique and characterized by using differential scanning calorimeter (dsc). thermal analysis also proved the formation of complex between sls & rho-123. the p values were found to be significant (<0.05) except group comprising 0.0001% sls,and that is because 0.0001% sls is seems to be very low to affect the solubility or complexation of rho-123.