Dimethyl 3,3',4,4'-tetrahydroxy-δ-truxinate isolated from the leaves of Andrographis lineata.Wall. Ex. Nees suppress adipogenesis in 3T3-L1 preadipocytes for type 2 diabetes

The present investigation elucidates the isolation and characterization of bioactive compound from the ethanolic leaf extract of andrographis lineata (etall) which suppress the differentiation of 3t3-l1 adipocytes. the ethanolic leaf extract was subjected to bioassay guided fractionation in 3t3-l1 cell lines. five fractions were isolated from the etall extract by column chromatography. all the fractions (i-v) along with etall were screened for adipogenesis activity (oil-red-o staining).the fraction which showed maximum adipogenesis activity was purified by thin layer chromatography. the bioactive fraction iv was found to have maximum adipogenic (96.83%) activity and the activity was comparable to rosiglitazone. the spectroscopic data analysis reveals that,the isolated bioactive compound was dimethyl 3,3',4,4'-tetrahydroxy-δ-truxinate (dtδt),a combination of truxillic and truxinic acid derivative. dtδt showed insulin mimicking (131.2%),sensitizing (810.02%) and adipogenic activity (80.23%). hence our present study concluded that,dimethyl 3,3',4,4'-tetrahydroxy-δ-truxinate isolated from the ethanolic leaf extract of andrographis lineata stimulates glucose uptake,potentiates insulin-stimulated glucose in 3t3-l1 adipocytes without increasing adiposity.