Exercise preconditioning reduces ischemia reperfusion-induced focal cerebral infarct volume through up-regulating the expression of HIF-1α

To study the effect and mechanism of exercise preconditioning on focal cerebral ischemia reperfusion induced cerebral infarction via rat model; sixty sprague dawley rats were divided into three groups at random: ischemia reperfusion group (ir,n=24),sham group (sham,n=12) and exercise preconditioning group (ep,n=24). group ep carried out moderate exercise preconditioning for 4 weeks (swimming with non-weight bearing,60 minutes/day,6 days/week),rats in group ep and ir were established cerebral ischemia reperfusion injury model by zea longa's thread method. the cerebral infarct volume in rat of different group was evaluated after 2%ttc staining,expression of hif-1α in rats' brain was detected by real-time rt-pcr,immunohistochmeistry method and western blot. no cerebral infarction and significant expression of hif-1α in group sham. compared with group ir,there was smaller infarct volume and stronger hif-1α expression in group ep (p<0.05). moderate exercise preconditioning reduces ischemia reperfusion induced focal cerebral infarct volume through up-regulating the expression of hif-1α.