Evaluation of anti-hyperglycemic effect of Actinidia kolomikta (Maxim. etRur.) Maxim. root extract

This study aimed to evaluate the anti-hyperglycemic effect of ethanol extract from actinidia kolomikta (maxim. etrur.) maxim. root (ake).an in vitro evaluation was performed by using rat intestinal α-glucosidase (maltase and sucrase),the key enzymes linked with type 2 diabetes. and an in vivo evaluation was also performed by loading maltose,sucrose,glucose to normal rats. as a result,ake showed concentration-dependent inhibition effects on rat intestinal maltase and rat intestinal sucrase with ic(50) values of 1.83 and 1.03mg/ml,respectively. in normal rats,after loaded with maltose,sucrose and glucose,administration of ake significantly reduced postprandial hyperglycemia,which is similar to acarbose used as an anti-diabetic drug. high contents of total phenolics (80.49 ± 0.05mg gae/g extract) and total flavonoids (430.69 ± 0.91mg re/g extract) were detected in ake. in conclusion,ake possessed anti-hyperglycemic effects and the possible mechanisms were associated with its inhibition on α-glucosidase and the improvement on insulin release and/or insulin sensitivity as well. the anti-hyperglycemic activity possessed by ake maybe attributable to its high contents of phenolic and flavonoid compounds.